Factors Affecting the Quality of Cryopreserved Dog Spermatozoa

Abstract
Limited research is done on canine semen as compared to dairy animals. Semen evaluation is very important to confirm the pregnancy in bitch and most of the factors are contributed by male, if used chilled or frozen. Dog’s health, semen collection procedure, use of extender, cooling and freezing time, thawing technique and insemination, all plays very important role while dealing with the breeding operation. Standardized procedures are to be adopted for successful results. This review covers the various observations pointed out by the scientists for semen handling during Artificial Insemination (AI).

Keywords
Semen evaluation, Dog Semen, Factors effecting semen quality, Successful AI in dogs

Cite this paper
Muhammad Ali Raza, Syed Murtaza Hussain Andrabi, Usman Ahmad, Kissa Zahra Baloch, Factors Affecting the Quality of Cryopreserved Dog Spermatozoa , SCIREA Journal of Animal Husbandry and Veterinary Medicine. Volume 6, Issue 1, February 2024 | PP. 1-10. 10.54647/ahvm230073

References

[ 1 ]	Alhaider AK and Watson PF. Cryopreservation of dog semen: The effects of Equex STM paste on plasma membrane fluidity and the control of intracellular free calcium. Animal Reproduction Science, 110(2009): 147-161,
[ 2 ]	Hewitt DA, Leahy R, Sheldon IM and England GCW. Cryopreservation of epididymal dog sperm. Animal Reprod Science. 67(2001): 101–111.
[ 3 ]	Hewitt DA, Leahy R, Sheldon IM and England GCW, Cryopreservation of epididymal dog sperm, Animal Reproduction Science, 67(2001): 101-111,
[ 4 ]	Domoslawska A, Zdunczyk S, Franczyk M, Kankofer M and Janowski T. Total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. Reprod. Domest. Anim., 54(2019): 252–257.
[ 5 ]	Lechner D, Aurich J, Schäfer-Somi S and Aurich C. Effects of age, size and season on cryotolerance of dog semen - A retrospective analysis. Anim. Reprod. Sci. (2022): 236
[ 6 ]	Woelders H. Fundamentals and recent development in cryopreservation of bull and boarsemen. Vet Quart., 19(1997):135–138.
[ 7 ]	Huebinger J. Modification of cellular membranes conveys cryoprotection to cells during rapid, non-equilibrium cryopreservation. 13(2018).
[ 8 ]	Hermansson U. Studies of Canine and Feline Sperm Viability under Different Storage Procedures. With special reference to chilling, freezing, and use of zona pellucida binding assays. Doctoral thesis. Swedish University of Agricultural Sciences Uppsala. 2006.
[ 9 ]	England GC, Millar KM. The ethics and role of AI with fresh and frozen semen in dogs. Reprod Domest Anim., 43(2008):165-7.
[ 10 ]	England GCW. Cryopreservation of dog semen: a review.J Reprod Fertil Suppl. 47(1993):243-55.
[ 11 ]	Manuela P and Birtoiu A. Freezing of dog's sperm: A review. Romanian Biotechnological, 17(2012): 7709-7716.
[ 12 ]	Oettle EE. Using a new acrosome stain to evaluate sperm morphology. Vet. Med. 81(1986): 263-266.
[ 13 ]	Songsasen N, Yu I, Leibo SP. Nuclear maturation of canine oocytes cultured in protein-free media. Mol Reprod Dev. 62(2002):407–415.
[ 14 ]	Petrunkina A, Volker, Gabriele, Brandt, Horst, Topfer-Petersen, Edda, Waberski and Dagmar. Functional significance of sperm responsiveness to capacitating conditions. Theriogenology. 64(2005). 1766-82.
[ 15 ]	Mazur P. Freezing live cells: Mechanisms and implications. Am. J. Physiol., 247(1984): 125-142.
[ 16 ]	Sirivaidyapong, Sudson, Ursem P, Bevers M, Colenbrander and Ben. Effect of prostatic fluid on motility, viability and acrosome integrity of chilled and frozen-thawed dog spermatozoa. Journal of reproduction and fertility. Supplement. 57(2001). 383-6.
[ 17 ]	Rijsselaere T, Soom AV, Maes D and Kruif AD. Effect of centrifugation on in vitro survival of fresh diluted canine spermatozoa. Theriogenology., 57(2002): 1669–1681.
[ 18 ]	Vieira, NDMG, de Agostini Losano, JD, Angrimani DDSR, Kawai GKV, de Cássia Bicudo L, Rui BR, da Silva BDCS, Assumpçao, MEODA and Nichi M. Induced sperm oxidative stress in dogs: Susceptibility against different reactive oxygen species and protective role of seminal plasma. Theriogenology, 108(2018): 39–45.
[ 19 ]	Schafer-Somi S, Colombo M and Luvoni GC. Canine Spermatozoa—Predictability of Cryotolerance. Animals, 12(2022): 733.
[ 20 ]	Nothling JO, Shuttleworth R, Haas KD, Thompson PN. Homologous prostatic fluidadded to frozen-thawed dog spermatozoa prior to intravaginal insemination of bitches resulted in better fertility then albumin-free TALP. Theriogenology., 64(2005): 975 – 991.
[ 21 ]	Schafer-Somi S and Palme N. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability. Reprod. Domest. Anim., 51(2016): 232–239.
[ 22 ]	Szasz F, Gabor G and Solti L. Comparative study of different methods for dog semen cryopreservation and testing under clinical conditions.Acta Vet Hung., 48(2000):325-333.
[ 23 ]	Silvia EC, Camila IV, Satzinger S, Ortiz ME, Assumpção DA and Visintin JA. Comparison of five extenders for canine semen freezing. Braz. J. Vet. Res. Anim. Sci. .36(1999): 5 – 10.
[ 24 ]	Linde-Forsberg C, Holst S, and Govette B. Comparison of fertility data from vaginal vs. intrauterine insemination of frozen-thawed dog semen: a retrospective study. Theriogenology 52(1999): 11–23.
[ 25 ]	Silva AR, Cardoso RCS, Uchoa DC and Silva LDM. Quality of canine semen submitted to single or fractionated glycerol addition during the freezing process, Theriogenology, 59(2003): 821-829.
[ 26 ]	England GCW. The cryopreservation of dog semen. London, England: University of London; (1992):1 - 124.
[ 27 ]	Bencharif D, Amirat I, Anton M, Schmitt E, Desherces S, Delhomme G, Langlois MI, Barrière P, Larrat M and Tainturier D. The advantages of LDL (Low Density Lipoproteins) in the cryopreservation of canine semen. Theriogenology., 70(2008): 1478 – 1488.
[ 28 ]	Rigau L, Carnprubi M, Badia J, Ballcster J, Palomo JM, Rivera M and lzquicrdo D. A. Peiia A and Rodrcguez-Gil JE. Different effects of glucose and fructose on energy metabolism in dog sperm from fresh ejaculates. 14th Intl Cong Anim Reprod., (2000): 224.
[ 29 ]	Olar TT, Bowen RA and Pickett BW. Influence of extender, cryopreservative and seminal processing procedures on post thaw motility on canine spermatozoa frozen in straws, Theriogenology., 31(1989): 451- 461.
[ 30 ]	Rota A, Linde-Forsberg C, Vanozzi I, Romagnoli S and Rodriguez-martinez H. Cryosurvival of dog spermatozoa at different glycerol concentrations and freezing-thawing rates, Reprod. Domest. Anim., 33(1998): 355 – 361.
[ 31 ]	Aurich C and Spergser J. Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa. Theriogenology, 67(2007): 912 – 918.
[ 32 ]	Peña AI, and Linde-forsberg C. Effects of Equex, one- or two- steps dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa. Theriogenology,53(2000): 859 – 875.
[ 33 ]	Hay MA, King WA, Gartley CJ, Leibo SP and Goodrowe KL. Canine spermatozoa -Cryopreservation and evaluation of gamete interaction. Theriogenology, 48(1997): 1329 – 1342.
[ 34 ]	Watson PF. Recent developments and concepts in cryopresenation of spem and assessment of their post-thawing function. Reprod. Fertïl. Dev., (1995)7: 87 1 -892,
[ 35 ]	Yu I, Songsasen N, Godke RA and Leibo SL. Differences among dogs in response of theirspermatozoa to cryopreservation using various cooling and warming rates. Cryobiology., 44(2002): 62 – 72.
[ 36 ]	Silva AR, R. D. Cardoso RDCS, Uchoa DC, Machado DA, Silva LD. Effect of tris-buffer, egg yolk and glycerol on canine semen freezing. Vet. J., 164(2002): 244 – 246.
[ 37 ]	Nothling JO and Shuttleworth R. The effect of straw size, freezing rate and thawing rate upon post-thaw quality of dog semen. Theriogenology.63(2003):1469–1480
[ 38 ]	Schafer SS, Kluger S, Knapp E, Klein D and Aurich C 2007. Effects of semen extender and semen processing on motility and membrane integrity of frozen-thawed dog spermatozoa, Theriogenology, 66(2007): 173–182.
[ 39 ]	Alamo D, Batista M, González F, Rodríguez N, Cruz G, Cabrera F and Gracia A. Cryopreservation of semen in the dog: use of ultra-freezers of -152C° as a viable alternative to liquidnitrogen. Theriogenology, 63(2005): 72 – 82.
[ 40 ]	Thomas PGA, Larsen RE, Burns JM and Hahn CN. A comparison of three packaging techniques using two extenders for the cryopreservation of canine semen. Theriogenology., 40(1993): 1199–1205.
[ 41 ]	Linde-Forsberg C. Artificial insemination with fresh, chilled-extended and frozen-thawed semen inthe dog, Sem. Vet. Med. Surg. (Small animal).,10(1995): 48 - 58.
[ 42 ]	Michael A, Alexopoulos C, Pontiki E, Hadjipavlou-Litina D and Saratsis P and Boscos C. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology., 68(2007): 204–212.